jak inhibitor group  (MedChemExpress)

Journal: BMC Pulmonary Medicine

Article Title: Transcriptomics reveals the underlying mechanism of Prostaglandin E1 in improving severe pneumonia

doi: 10.1186/s12890-025-03847-y

Figure Lengend Snippet: PGE1 participates in severe pneumonia by regulating JAK-STAT pathway. A Volcano plot showed the DEGs between the LPS and LPS + PGE1 groups; B Bubble plot from KEGG and GO analyses showed the major enrichment of up-regulated DEGs; C Bubble plot from KEGG and GO analyses showed the major enrichment of down-regulated DEGs; D GSEA-KEGG showed the enrichment of gene-related signaling pathways; E Heatmap showed JAK-STAT pathway-related gene expression between the two groups

Article Snippet: The tongue and the nose were loosened after 20 s, and then the mice were placed in a cage and allowed to awaken naturally to construct severe pneumonia models [ ]; (3) PGE1 intervention group (LPS + PGE1): after LPS treatment, the mice were also administered 10 mg/kg PGE1 intraperitoneally once a day for 4 d; (4) Dimethyl sulfoxide (DMSO) group (LPS + PGE1 + DMSO): After LPS and PGE1 treatment, the mice were also injected intraperitoneally with an equal dose of 0.1% DMSO in PBS solution; (5) JAK inhibitor group (LPS + PGE1 + AG490): After LPS and PGE1 treatment, the mice were also intraperitoneally injected with 1 mg/kg AG490 (HY-12000, MedChemExpress).

Techniques: Protein-Protein interactions, Gene Expression

Journal: BMC Pulmonary Medicine

Article Title: Transcriptomics reveals the underlying mechanism of Prostaglandin E1 in improving severe pneumonia

doi: 10.1186/s12890-025-03847-y

Figure Lengend Snippet: Combination of PGE1 and JAK inhibitor can regulate the phenotype of mice with severe pneumonia. A H&E staining showed the pathological changes in mouse lung tissues; B W/D values of mouse lung tissues; C ELISA showed the levels of IL-1β, IL-6, IL-10, and TNF-α in mouse BALF and lung tissues; D The number of WBCs and PMNs in mouse BALF; and E Blood gas analyzer detected the PaO 2 and PaCO 2 of mice. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The tongue and the nose were loosened after 20 s, and then the mice were placed in a cage and allowed to awaken naturally to construct severe pneumonia models [ ]; (3) PGE1 intervention group (LPS + PGE1): after LPS treatment, the mice were also administered 10 mg/kg PGE1 intraperitoneally once a day for 4 d; (4) Dimethyl sulfoxide (DMSO) group (LPS + PGE1 + DMSO): After LPS and PGE1 treatment, the mice were also injected intraperitoneally with an equal dose of 0.1% DMSO in PBS solution; (5) JAK inhibitor group (LPS + PGE1 + AG490): After LPS and PGE1 treatment, the mice were also intraperitoneally injected with 1 mg/kg AG490 (HY-12000, MedChemExpress).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: BMC Pulmonary Medicine

Article Title: Transcriptomics reveals the underlying mechanism of Prostaglandin E1 in improving severe pneumonia

doi: 10.1186/s12890-025-03847-y

Figure Lengend Snippet: Combination of PGE1 and JAK inhibitor can regulate Th17/Treg balance in mice with severe pneumonia. A Flow cytometry detected the ratios of Th17 and Treg cells; B qPCR detected RORγt and Foxp3 mRNA expression. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The tongue and the nose were loosened after 20 s, and then the mice were placed in a cage and allowed to awaken naturally to construct severe pneumonia models [ ]; (3) PGE1 intervention group (LPS + PGE1): after LPS treatment, the mice were also administered 10 mg/kg PGE1 intraperitoneally once a day for 4 d; (4) Dimethyl sulfoxide (DMSO) group (LPS + PGE1 + DMSO): After LPS and PGE1 treatment, the mice were also injected intraperitoneally with an equal dose of 0.1% DMSO in PBS solution; (5) JAK inhibitor group (LPS + PGE1 + AG490): After LPS and PGE1 treatment, the mice were also intraperitoneally injected with 1 mg/kg AG490 (HY-12000, MedChemExpress).

Techniques: Flow Cytometry, Expressing